Cite this as: Haponava, V., Fibiger. L., Butler, I.B. and Pickard, C. 2025 Towards Responsible Destructive Analysis: A guide to the recording of archaeological tooth samples with laboratory process visualisation, Internet Archaeology 69. https://doi.org/10.11141/ia.69.4
With increasing use of destructive analysis in archaeology, a tension has arisen between the need to preserve osteological collections for future generations and to use them now for the public benefit of knowledge generation. Existing guidelines mostly address curatorial issues, or focus on pre-sampling steps, rather than presenting protocols that can assist researchers in being more responsible regarding invasive analysis and the preservation of osteological collections. This article therefore presents guidelines for the recording of archaeological tooth samples prior to destructive analysis in the form of a process diagram accompanied by written instructions. The aim is twofold: to promote good practice in preserving a record of a sample before its destructive analysis, and to provide accessible material that can be used in lectures or training to assist students in visualising common laboratory processes in the field of bioarchaeology, as well as for public outreach and knowledge exchange. The diagram is enriched with laboratory videos for each step, and should help demystify the laboratory process for general audiences.
Corresponding author: Vera Haponava
harti@protonmail.com
University of Edinburgh
Linda Fibiger
University of Edinburgh
Ian B. Butler
University of Edinburgh
Catriona Pickard
University of Edinburgh
Figure 1: Diagram of the sample preparation process, including the steps for preserving a record of the sample and unused tissue (highlighted in bold), and collagen and carbonate processing.
Presentation: The laboratory process for stable isotope analysis of dentine, including steps for sample preservation, and collagen and carbonate stable isotope analyses.
Video: A video showing the steps described in the Presentation: sample preservation, and collagen and carbonate stable isotope analyses. The video was recorded using Debut (NCH Software) and Prezi for Desktop applications.
AlQahtani, S.J., Hector, M.P. and Liversidge, H.M. 2010 'Brief communication: The London atlas of human tooth development and eruption', American Journal of Physical Anthropology 142, 481–90. https://doi.org/10.1002/ajpa.21258
Antoine, D. and Ambers, J. 2014 'The Scientific Analysis of Human Remains from the British Museum Collection: Research Potential and Examples from the Nile Valley' in A. Fletcher, D. Antoine and J. Hill (eds) Regarding the Dead: Human Remains in the British Museum, British Museum Press: London. 20–30
Austin, R.M., Sholts, S.B., Williams, L., Kistler, L. and Hofman, C.A.2019 'To curate the molecular past, museums need a carefully considered set of best practices', Proceedings of the National Academy of Sciences 116, 1471–4. https://doi.org/10.1073/pnas.1822038116
Avery, L.C., Brickley, M.B., Findlay, S., Bondioli, L., Sperduti, A. and Prowse, T.L. 2023 'Eating Like adults: an investigation of dietary change in childhood and adolescence at Portus Romae (Italy, first–fourth centuries C.E.)', Bioarchaeology International 7, 130–46. https://doi.org/10.5744/bi.2022.0006
BABAO 2019 Code of Practice, British Association for Biological Anthropology and Osteoarchaeology. https://babao.org.uk/resources/guidelines-codes/
Balasse, M. 2002 'Reconstructing dietary and environmental history from enamel isotopic analysis: time resolution of intra-tooth sequential sampling', International Journal of Osteoarchaeology 12, 155–65. https://doi.org/10.1002/oa.601
Beaumont, J., Gledhill, A., Lee-Thorp, J. and Montgomery, J. 2013 'Childhood diet: a closer examination of the evidence from dental tissues using stable isotope analysis of incremental human dentine', Archaeometry 55, 277–95. https://doi.org/10.1111/j.1475-4754.2012.00682.x
Brickley, M. and McKinley, J.I. 2004 Guidelines to the Standards for Recording Human Remains, BABAO, Department of Archaeology and the Institute of Field Archaeologists. Guidelines to the Standards for Recording Human Remains. https://babao.org.uk/resources/guidelines-codes/
Cheung, C., Fernández-Crespo, T., Mion, L., Di Giusto, M., Goude, G., Macdonald, R.A., Richards, M.P. and Herrscher, E. 2022 'Micro-punches versus micro-slices for serial sampling of human dentine: striking a balance between improved temporal resolution and measuring additional isotope systems', Rapid Communications in Mass Spectrometry 36, e9380. https://doi.org/10.1002/rcm.9380
Coutsiers Morell, G.F., Berlin-Broner, Y., Flores-Mir, C. and Heo, G. 2022 'Tooth and root size as determined from 0.25- and 0.30-mm voxel size cone-beam CT imaging when contrasted to micro-CT scans (0.06 mm): An ex vivo study', Journal of Orthodontics 49, 174–8. https://doi.org/10.1177/14653125211066106
Curtis, M.J., Beaumont, J., Elamin, F., Wilson, A.S. and Koon, H.E.C. 2022 'Method of micro-sampling human dentine collagen for stable isotope analysis', Rapid Communications in Mass Spectrometry 36, e9305. https://doi.org/10.1002/rcm.9305
Czermak, A., Fernández-Crespo, T., Ditchfield, P.W. and Lee-Thorp, J.A. 2020 'A guide for an anatomically sensitive dentine microsampling and age-alignment approach for human teeth isotopic sequences', American Journal of Physical Anthropology 173, 776–83. https://doi.org/10.1002/ajpa.24126
Elders, J., Humphrey, L., Mays, S. and White, W. 2011 Sampling Human Remains for Ancient DNA analysis. APABE Supplementary Guidance Note 1, Advisory Panel on the Archaeology of Burials in England.
Eriksson, G. 2013 'Stable Isotope Analysis of Humans' in L.N. Stutz and S. Tarlow (eds) The Oxford Handbook of the Archaeology of Death and Burial, Oxford University Press: Oxford. 123–46. https://doi.org/10.1093/oxfordhb/9780199569069.013.0008
Fiorenza, L., Benazzi, S. and Kullmer, O. 2009 'Morphology, wear and 3D digital surface models: materials and techniques to create high-resolution replicas of teeth', Journal of Anthropological Science 87, 211–18
Galbany, J., Martínez, L.M. and Pérez-Pérez, A. 2004 'Tooth replication techniques, sem imaging and microwear analysis in primates: methodological obstacles', Anthropologie 42, 5–12
Hendy, J., Welker, F., Demarchi, B., Speller, C., Warinner, C. and Collins, M.J. 2018 'A guide to ancient protein studies', Nature Ecology and Evolution 2, 791–9. https://doi.org/10.1038/s41559-018-0510-x
Hillson, S.W. 1992 'Impression and replica methods for studying hypoplasia and perikymata on human tooth crown surfaces from archaeological sites', International Journal of Osteoarchaeology 2, 65–78. https://doi.org/10.1002/oa.1390020109
Hillson, S. 1996 Dental Anthropology, Cambridge University Press: Cambridge/New York.
Hillson, S. 2001 'Recording dental caries in archaeological human remains', International Journal of Osteoarchaeology 11, 249–89. https://doi.org/10.1002/oa.538
Hillson, S. 2008 'Dental Pathology' in M. A. Katzenberg and S. R. Saunders (eds) Biological Anthropology of the Human Skeleton, John Wiley & Sons: Chichester. 299–340. https://doi.org/10.1002/9780470245842.ch10
Hillson, S. 2023 Dental Anthropology, 2nd edn, Cambridge University Press: Cambridge. https://doi.org/10.1017/9781108381550
Human Remains Working Group 2013 Recommendations for the Care of Human Remains in Museums and Collections, Human Remains Working Group
Immel, A., Le Cabec, A., Bonazzi, M., Herbig, A., Temming, H., Schuenemann, V.J., Bos, K.I., Langbein, F., Harvati, K., Bridault, A., Pion, G., Julien, M.-A., Krotova, O., Conard, N.J., Münzel, S.C., Drucker, D.G., Viola, B., Hublin, J.-J., Tafforeau, P. and Krause, J. 2016 'Effect of X-ray irradiation on ancient DNA in sub-fossil bones – guidelines for safe X-ray imaging', Scientific Reports 6. https://doi.org/10.1038/srep32969
Keller, M. and Scheib, C.L. 2023 Sampling of Tooth Roots for Ancient DNA, Springer, protocols.io. https://doi.org/10.17504/protocols.io.6qpvrdr83gmk/v1
Mays, S., Elders, J., Humphrey, L., White, W. and Marshall, P. 2023 Science and the Dead: Destructive Sampling of Archaeological Human Remains for Scientific Analysis, 2nd edn, Historic England, Advisory Panel on the Archaeology of Burials in England.
Pálsdóttir, A.H., Bläuer, A., Rannamäe, E., Boessenkool, S. and Hallsson, J.H. 2019 'Not a limitless resource: ethics and guidelines for destructive sampling of archaeofaunal remains', Royal Society Open Science 6, 191059. < href="https://doi.org/10.1098/rsos.191059" target="external">https://doi.org/10.1098/rsos.191059
Parker, C.E., Bos, K.I., Haak, W. and Krause, J. 2021 'Optimized bone sampling protocols for the retrieval of ancient DNA from archaeological remains', JoVE, 63250. https://doi.org/10.3791/63250
Richards, M.P. and Hedges, R.E.M. 1999 'Stable isotope evidence for similarities in the types of marine foods used by late Mesolithic humans at sites along the Atlantic coast of Europe', Journal of Archaeological Science 26, 717–22. https://doi.org/10.1006/jasc.1998.0387
Roberts, C.A. 2016 'Palaeopathology and its relevance to understanding health and disease today: the impact of the environment on health, past and present', Anthropological Review 79, 1–16. https://doi.org/10.1515/anre-2016-0001
Roberts, P., Fernandes, R., Craig, O.E., Larsen, T., Lucquin, A., Swift, J. and Zech, J. 2018 'Calling all archaeologists: guidelines for terminology, methodology, data handling, and reporting when undertaking and reviewing stable isotope applications in archaeology', Rapid Communications in Mass Spectrometry 32, 361–372. https://doi.org/10.1002/rcm.8044
Sabin, S. and Yates, J.A.F. 2020 Dental Calculus Field-Sampling Protocol (Sabin version), Springer, protocols.io. https://doi.org/10.17504/protocols.io.bqecmtaw
Sealy, J., Johnson, M., Richards, M. and Nehlich, O. 2014 'Comparison of two methods of extracting bone collagen for stable carbon and nitrogen isotope analysis: comparing whole bone demineralization with gelatinization and ultrafiltration', Journal of Archaeological Science 47, 64–69. https://doi.org/10.1016/j.jas.2014.04.011
Smith, B.H. 1984 'Patterns of molar wear in hunter–gatherers and agriculturalists', American Journal of Physical Anthropology 63, 39–56. https://doi.org/10.1002/ajpa.1330630107
Smithsonian National Museum of Natural History 2018 Anthropology Sampling Review Committee Policies, Procedures and Guidelines, Smithsonian National Museum of Natural History, Washington DC
Squires, K., Booth, T., Roberts, C.A., 2019 'The Ethics of Sampling Human Skeletal Remains for Destructive Analyses' in K. Squires, D. Errickson and N. Márquez-Grant (eds) Ethical Approaches to Human Remains: A Global Challenge in Bioarchaeology and Forensic Anthropology, Springer: Cham. 265–97. https://doi.org/10.1007/978-3-030-32926-6_12
Steckel, R.H., Larsen, C.S., Sciulli, P.W. and Walker, P.L. 2018 'Data Collection Codebook' in R.H. Steckel, C.S. Larsen, C.A. Roberts and J. Baten (eds) The Backbone of Europe: Health, Diet, Work and Violence over Two Millennia, Cambridge: Cambridge University Press (Cambridge Studies in Biological and Evolutionary Anthropology), 397–427. https://doi.org/10.1017/9781108379830.016
University of Edinburgh 2024 'Statement of Principles', University of Edinburgh: Edinburgh. https://hca.ed.ac.uk/research/ethics-procedures/principles (Last accessed: 11 November 2024)
Ventresca Miller, A., Fernandes, R., Janzen, A., Nayak, A., Swift, J., Zech, J., Boivin, N. and Roberts, P. 2018 'Sampling and pretreatment of tooth enamel carbonate for stable carbon and oxygen isotope analysis', JoVE, 58002. https://doi.org/10.3791/58002
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